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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
Software Version 5.06, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with <t>GraFit</t> <t>software.</t> ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .
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Image Search Results


DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with GraFit software. ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .

Journal: International Journal of Molecular Sciences

Article Title: Disulfiram/Copper Suppresses Cancer Stem Cell Activity in Differentiated Thyroid Cancer Cells by Inhibiting BMI1 Expression

doi: 10.3390/ijms232113276

Figure Lengend Snippet: DSF/copper inhibits cell proliferation and thyrosphere formation of DTC cells. ( A , B ) K1 ( A ) or WRO ( B ) cells were seeded into 96-well-plates and treated with the indicated concentrations of DSF in presence of 1 μM CuCl 2 for 72 h. The cell proliferation was determined by WST-1 reagent and the absorbance was read at 440 nm wavelength. The curves and IC 50 values were drawn and calculated with GraFit software. ( C , D ) K1 ( C ) or WRO ( D ) cells were seeded into ultralow attachment culture dishes to form primary thyrospheres for 7 days under the treatment of indicated concentrations of DSF in presence of 1 μM CuCl 2 . The formed thyrospheres were pictured and counted on Day 7. *, p < 0.05; **, p < 0.01. Scale bars in ( C , D ) represented as 50 nm. ( E ) The thyrospheres from K1 or WRO cells were dissociated into single-cell suspensions by enzyme-free cell dissociation buffer and treated with 200 nM of DSF in presence of 1 μM CuCl 2 for 24 h. The expression of CD44 was determined by flow cytometry. Data were analyzed by FlowJo software. The dotted arrows indicate the percentage of CD44 high expression cells that with the fluorescence intensity greater than 10 4 .

Article Snippet: The IC 50 values were calculated by GraFit (version 5.0.6, Erithacus Software, West Sussex, UK).

Techniques: Software, Expressing, Flow Cytometry, Fluorescence